SUMseq article published in Nature Methods
Author: Annique Claringbould
🧬 Our method SUM-seq for joint profiling of single-cell mRNA and chromatin accessibility (ATAC) is now published in Nature Methods!
🚀 By combining combinatorial indexing with droplet-based barcoding, SUM-seq enables large-scale multiplexing with improved data quality.
We applied this method to three different use cases and found that SUM-seq
- Captures gene regulatory dynamics during macrophage polarization
- Reveals transcriptional programs underlying differentiation of T helper cell subtypes
- Enables dissection of transcription factor function using CRISPRi/a screens during iPSC differentiation
This work was the result of a big collaborative effort among members of Judith Zaugg and Kyung-Min Noh labs, specifically Sara Lobato-Moreno, Umut Yildiz, Mikael Marttinen, and other great collaborators!
